Ilirjana Boci – Department of Industrial Chemistry, Faculty of Natural Sciences, UT, Blv ZOG 1, Tirana, Albania

Keywords:                     Nitrite analysis;
Spectrophotometry methods;
Calibration curves;


Abstract: In most countries, the use of nitrite in meat processed products ei­ther ingoing or the residual amount, is regulated by law. This regulation is continuously updated reflecting the newest and most relevant data after bet­ter understanding nitrite’s potential harmful effects and its transformation pathways in organisms. Sodium nitrite added to meat products is partly con­verted to the heat stable NO-myoglobin and to nitrate by oxidation, acting this way as an antioxidant. These biochemical red ox reactions are not ful­ly understood because they depend on many variables in meat matrixes. Ni­trite residual content is limited at lower than 100 mg/kg product for most of the meat processed products, but because of the abovementioned biochemi­cal often simultaneous reactions, its monitoring should be continuous based on a scientific sampling methodology and accurate methods of analysis. This paper aims to compare two UV-Vis spectrophotometric methods against the reference ISO method for nitrite analysis to recommend their use in specific cases. These methods mainly differ in the nitrite extraction procedures from the meat matrices. The SF UV-Vis AOAC method based on nitrite extraction in natural meat sample conditions and the method proposed by Merino L. in al­kaline conditions are compared to the ISO method by plotting the interaction graph of the data obtained. The results obtained are satisfactory and the au­thor recommends the Merino method when nitrate is to be analyzed in the same sample, otherwise, the AOAC method would be the choice as not much sample handling is required.

9th International Scientific ERAZ Conference – ERAZ 2023 – Conference Proceedings: KNOWLEDGE BASED SUSTAINABLE DEVELOPMENT, hybrid – online, virtually and in person, Prague, Czech Republic, June 1, 2023

ERAZ Conference Proceedings published by: Association of Economists and Managers of the Balkans – Belgrade, Serbia

ERAZ conference partners: Faculty of Logistics, University of Maribor, Maribor (Slovenia); University of National and World Economy – UNWE, Sofia (Bulgaria); Center for Political Research and Documentation (KEPET), Research Laboratory of the Department of Political Science of University of Crete (Greece); Institute of Public Finance – Zagreb (Croatia); Faculty of Tourism and Hospitality Ohrid, University of St. Kliment Ohridski from Bitola (North Macedonia)

ERAZ Conference 2023 Conference Proceedings: ISBN 978-86-80194-72-1, ISSN 2683-5568, DOI:

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 License ( which permits non-commercial use, reproduction and distribution of the work without further permission. 

Suggested citation

Boci, I. (2023). Comparison of Two Nitrite UV-VIS Spectrophotometric Analysis Methods in Meat Processed Product. In V. Bevanda (Ed.), ERAZ Conference – Knowlegde Based Sustainable Development: Vol 9. Conference Proceedings (pp. 567-574). Association of Economists and Managers of the Balkans.


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